5ZG3
Crystal structure of the GluA2o LBD in complex with glutamate and TAK-137
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976486 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.065, 162.332, 47.385 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.650 |
| R-factor | 0.18571 |
| Rwork | 0.184 |
| R-free | 0.21486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.339 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 205163 | 10200 |
| <I/σ(I)> | 19 | 1.5 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.9 | 277 | 15% PEG 3350, 0.1M SODIUM ACETATE, 0.1M ZINC ACETATE |
