5ZFY

Crystal structure of human DUX4 homeodomains bound to A12C DNA mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977776
Spacegroup name	P 32 2 1
Unit cell lengths	65.256, 65.256, 150.697
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.941 - 2.300
R-factor	0.1863
Rwork	0.185
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z6z
RMSD bond length	0.004
RMSD bond angle	0.663
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	17918
<I/σ(I)>	22.95
Completeness [%]	99.6
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	0.1 M Sodium malonate pH 4.0, 12% Polyethylene glycol 3350