5ZF6
Crystal structure of the dimeric human PNPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.335, 109.334, 84.501 |
| Unit cell angles | 90.00, 117.41, 90.00 |
Refinement procedure
| Resolution | 42.157 - 2.796 |
| R-factor | 0.1967 |
| Rwork | 0.193 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u1k |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.157 | 2.890 |
| High resolution limit [Å] | 2.796 | 2.796 |
| Rmerge | 0.081 | 0.570 |
| Number of reflections | 32471 | 1594 |
| <I/σ(I)> | 15.03 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1M citrate buffer (pH 5.0), 10% (v/v) 2-propanol, 26% (v/v) polyethylene glycol 400 |
