5ZBZ

Crystal structure of the DEAD domain of Human eIF4A with sanguinarine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-12-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979253
Spacegroup name	C 1 2 1
Unit cell lengths	73.471, 56.630, 66.324
Unit cell angles	90.00, 116.78, 90.00

Refinement procedure

Resolution	36.492 - 1.309
R-factor	0.194325913427
Rwork	0.194
R-free	0.21042
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zu6
RMSD bond length	0.009
RMSD bond angle	1.251
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.490	1.355
High resolution limit [Å]	1.309	1.309
Rmerge	0.027	0.318
Rmeas	0.038	0.450
Rpim	0.027	0.318
Number of reflections	53159	5697
<I/σ(I)>	12.23	2.21
Completeness [%]	90.7	97.52
Redundancy	2	1.9
CC(1/2)	0.995	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium malonate