5YQZ

Structure of the glucagon receptor in complex with a glucagon analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	60.120, 108.790, 216.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.590 - 3.000
R-factor	0.233
Rwork	0.232
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xez 4lf3 2rh1
RMSD bond length	0.009
RMSD bond angle	1.050
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	28879
<I/σ(I)>	21.6
Completeness [%]	97.8
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8	293	100 mM Tris, pH 8.0, 70-120 mM potassium phosphate dibasic, 27-33% (v/v) PEG 200