5YL6
Crystal structure of Ofd2 in complex with 2OG from Schizosaccharomyces pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2014-11-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.114, 80.312, 82.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.580 - 2.000 |
| R-factor | 0.2026 |
| Rwork | 0.200 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3thp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.091 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.580 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.060 |
| Number of reflections | 15373 |
| <I/σ(I)> | 2.4 |
| Completeness [%] | 99.9 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 18% MPEG 5000, 0.1M HEPES, pH 7.4 |
