5YIZ
Mouse Cereblon thalidomide binding domain complexed with racemic thalidomide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-17 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 |
Unit cell lengths | 201.376, 201.376, 123.358 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.060 - 2.000 |
R-factor | 0.18541 |
Rwork | 0.184 |
R-free | 0.21252 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wx2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.961 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 125695 |
<I/σ(I)> | 20.6 |
Completeness [%] | 100.0 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.0M ammonium sulfate 0.1M sodium acetate (pH5.0) |