5YHW
Crystal structure of Pig SAMHD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-12 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.540, 95.909, 130.749 |
| Unit cell angles | 78.66, 88.12, 82.68 |
Refinement procedure
| Resolution | 29.944 - 2.700 |
| R-factor | 0.2635 |
| Rwork | 0.263 |
| R-free | 0.29040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.659 |
| Data reduction software | PHENIX ((1.10.1_2155)) |
| Data scaling software | PHENIX ((1.10.1_2155)) |
| Phasing software | PHENIX ((1.10.1_2155)) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.787 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 108746 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 96.9 | 96.1 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Sodium acetate trihydrate pH 7.0, 20% w/v Polyethylene glycol 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
