5YE4
Crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.346, 164.093, 73.700 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 37.761 - 1.799 |
| R-factor | 0.1944 |
| Rwork | 0.194 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fj1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 287775 | |
| <I/σ(I)> | 28.3 | |
| Completeness [%] | 99.0 | 96.8 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM imidazole (pH 6.5), 150 mM zinc acetate, and 17.5% (w/v) PEG3000 |
