5Y7X
Human Peroxisome proliferator-activated receptor (PPAR) delta in complexed with a potent and selective agonist
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.766, 93.896, 96.871 |
Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
Resolution | 29.655 - 1.699 |
R-factor | 0.2061 |
Rwork | 0.205 |
R-free | 0.23050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u3r |
RMSD bond length | 0.006 |
RMSD bond angle | 0.790 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.800 | 1.760 |
High resolution limit [Å] | 1.699 | 1.700 |
Rmerge | 0.066 | 0.963 |
Number of reflections | 76750 | |
<I/σ(I)> | 32.5 | |
Completeness [%] | 98.9 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10% PEG 8000, 40mM Bis-Tris propane pH 8.5, 2.5% 1,2-propanediol, 200mM KCl, 10mM DTT |