5Y6F

Crystal structure of YcgR in complex with c-di-GMP from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	H 3
Unit cell lengths	93.980, 93.980, 109.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.990 - 2.300
R-factor	0.1855
Rwork	0.183
R-free	0.23340
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.380
Data reduction software	iMOSFLM (v.7.0.4)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.990	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.493
Rpim	0.026	0.232
Number of reflections	15972	2357
<I/σ(I)>	15	3.1
Completeness [%]	99.5	100
Redundancy	5.4	5.5
CC(1/2)	0.998	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.2M potassium thiocyanate, 0.1M Bis-Tris pH 6.0, 20% (w/v) PEG 3350