5Y3K
Crystal structure of horse TLR9 in complex with two DNAs (CpG DNA and GCGCAC DNA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.849, 126.502, 140.716 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.700 |
| R-factor | 0.20739 |
| Rwork | 0.205 |
| R-free | 0.24552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wpc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.140 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 50239 |
| <I/σ(I)> | 12 |
| Completeness [%] | 98.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4-6%(w/v) PEG 4000, 4-6%(v/v) 2-propanol, 0.2M NaCl, 0.1M citrate-NaoH pH 5.6 |
