5XJL

Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human

Replaces:  3S6N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99993
Spacegroup name	P 21 21 21
Unit cell lengths	82.830, 84.600, 104.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.190 - 2.500
R-factor	0.2238
Rwork	0.220
R-free	0.29570
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	59.190
High resolution limit [Å]	2.500
Number of reflections	19831
<I/σ(I)>	13.7
Completeness [%]	99.3
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	1%PEG8000, 100mM Tris-HCl