5XIT
Crystal structure of RNF168 UDM1 in complex with Lys63-linked diubiquitin, form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-10-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.372, 50.019, 64.407 |
| Unit cell angles | 73.49, 69.69, 73.82 |
Refinement procedure
| Resolution | 46.986 - 2.250 |
| R-factor | 0.2213 |
| Rwork | 0.220 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fid |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 21397 | |
| <I/σ(I)> | 20.2 | 1.8 |
| Completeness [%] | 90.2 | 91.5 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 23% PEG MME 2000, 0.1 M Bis-Tris (pH 6.5), 10 mM Pr acetate |
