5XHC
Crystal structure of T2R-TTL-PO10 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.257, 157.885, 182.427 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 119.380 - 2.750 |
R-factor | 0.22993 |
Rwork | 0.227 |
R-free | 0.27682 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fnv |
RMSD bond length | 0.007 |
RMSD bond angle | 1.237 |
Data reduction software | SCALEPACK |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 119.380 |
High resolution limit [Å] | 2.750 |
Number of reflections | 78443 |
<I/σ(I)> | 0 |
Completeness [%] | 99.8 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |