5XH6

Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RVR variant in complex with crRNA and target DNA (TATA PAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	81.162, 133.676, 199.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.990 - 2.000
R-factor	0.1767
Rwork	0.175
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b43
RMSD bond length	0.007
RMSD bond angle	0.843
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.990	2.023
High resolution limit [Å]	2.000	2.000
Rpim		0.396
Number of reflections	143997
<I/σ(I)>	17.1
Completeness [%]	98.2
Redundancy	6.6
CC(1/2)		0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7-10% PEG 3350, 100mM sodium acetate, pH 4.5, 10% 1,6-hexanediol