5XH6
Crystal structure of the Acidaminococcus sp. BV3L6 Cpf1 RVR variant in complex with crRNA and target DNA (TATA PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cpf1 | polymer | 1310 | 151784.4 | 1 | UniProt (U2UMQ6) | Acidaminococcus sp. (strain BV3L6) | AsCpf1 |
| 2 | B (B) | crRNA | polymer | 43 | 13756.1 | 1 | Acidaminococcus sp. BV3L6 | ||
| 3 | C (C) | Target DNA strand | polymer | 34 | 10434.7 | 1 | Acidaminococcus sp. BV3L6 | ||
| 4 | D (D) | Non-target DNA strand | polymer | 10 | 3004.0 | 1 | Acidaminococcus sp. BV3L6 | ||
| 5 | E, R, S, T, Y (A, B, C) | SODIUM ION | non-polymer | 23.0 | 5 | Chemie (NA) | |||
| 6 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | AA, BA, G, H, I... (C, A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 18 | Chemie (EDO) | |||
| 8 | CA, DA, EA, FA (A, B, C, D) | water | water | 18.0 | 659 | Chemie (HOH) |
Sequence modifications
A: 1 - 1307 (UniProt: U2UMQ6)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Arg 542 | Ser 542 | engineered mutation |
| Val 548 | Lys 548 | engineered mutation |
| Arg 552 | Asn 552 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 178979.2 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 1267.6 | |
| All* | Total formula weight | 180246.9 |
