5X68
Crystal Structure of Human KMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2012-12-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 176.736, 176.736, 89.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 88.370 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.666 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.370 | |
| High resolution limit [Å] | 2.100 | |
| Rmerge | 0.060 | 0.379 |
| Number of reflections | 55790 | |
| <I/σ(I)> | 25.6 | 2.25 |
| Completeness [%] | 96.5 | |
| Redundancy | 3.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris (pH 8.5), 13% polyethylene glycol 4000, and 15% glycerol |
