5X2A

Crystal structure of EGFR 696-1022 T790M/V948R in complex with SKLB(3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97924
Spacegroup name	P 1 21 1
Unit cell lengths	72.185, 103.973, 87.150
Unit cell angles	90.00, 101.40, 90.00

Refinement procedure

Resolution	39.487 - 1.850
R-factor	0.1758
Rwork	0.173
R-free	0.21940
RMSD bond length	0.023
RMSD bond angle	1.890
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Number of reflections	105418
<I/σ(I)>	14.6
Completeness [%]	98.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Bis-Tris 5.0, 22.5% PEG 3350, 5mM TCEP