5WY8

Crystal structure of PTP delta Ig1-Ig3 in complex with IL1RAPL1 Ig1-Ig3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 32 1 2
Unit cell lengths	110.416, 110.416, 210.536
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.892 - 3.070
R-factor	0.2341
Rwork	0.233
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.976
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.094	0.048	0.571
Total number of observations	223878
Number of reflections	28613	2987	2692
<I/σ(I)>	11.7
Completeness [%]	97.9	97.9	93.8
Redundancy	7.8	8.7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	100mM Tris-Cl, PH7.5, 200mM zinc acetate, 10% PEG 8000(v/v)