5WY0

Crystal structure of the methyltranferase domain of human HEN1 in complex with AdoMet

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-21
Detector	RAYONIX MX-225
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	38.521, 38.521, 249.437
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.731 - 2.001
R-factor	0.2077
Rwork	0.203
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.138
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Number of reflections	15433
<I/σ(I)>	15.9
Completeness [%]	98.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		290	0.1 M Tris pH 7.5, 0.2 M Magnesium chloride, 10% (w/v) PEG 3350