5WY0
Crystal structure of the methyltranferase domain of human HEN1 in complex with AdoMet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 38.521, 38.521, 249.437 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.731 - 2.001 |
| R-factor | 0.2077 |
| Rwork | 0.203 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.138 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 15433 |
| <I/σ(I)> | 15.9 |
| Completeness [%] | 98.9 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 290 | 0.1 M Tris pH 7.5, 0.2 M Magnesium chloride, 10% (w/v) PEG 3350 |
