5WUK

Crystal structure of EED [G255D] in complex with EZH2 peptide and EED226 compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-15
Detector	MAR CCD 130 mm
Wavelength(s)	0.97845
Spacegroup name	P 21 21 21
Unit cell lengths	53.100, 80.280, 108.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 2.030
R-factor	0.162
Rwork	0.160
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.040
Data scaling software	SCALEPACK
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	64.520
High resolution limit [Å]	2.030
Number of reflections	30739
<I/σ(I)>	5
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% polyethylene glycol (PEG) 6000, 0.1M Bicine(pH9.0)