5WLW

Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	97.529, 121.241, 151.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.764 - 3.317
R-factor	0.2005
Rwork	0.196
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lmv 3lvl 2fae
RMSD bond length	0.002
RMSD bond angle	0.579
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	3.520
High resolution limit [Å]	3.300	3.300
Number of reflections	27282
<I/σ(I)>	13.7	2.73
Completeness [%]	99.8	99.1
Redundancy	13.7
CC(1/2)	0.997	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1 M MES pH 7.0 15 % PEG 3350