5WKP

Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	98.360, 123.290, 151.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.389 - 3.150
R-factor	0.1897
Rwork	0.186
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lvm 3lvl 2fae
RMSD bond length	0.002
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.389
High resolution limit [Å]	3.150
Number of reflections	32516
<I/σ(I)>	15.4
Completeness [%]	99.8
Redundancy	9.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	285	0.1 M MES pH 7 15 % PEG 3350