5WIS

Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	209.620, 448.780, 622.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	255.767 - 2.703
R-factor	0.2242
Rwork	0.222
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.822
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	255.767	255.767	2.770
High resolution limit [Å]	2.700	12.090	2.700
Rmerge	0.142	0.019	1.185
Rmeas	0.168	0.022	1.406
Number of reflections	1561494	17891	110459
<I/σ(I)>	8.22	37.04	1.06
Completeness [%]	98.9	95.7	95
Redundancy	3.408	3.27	3.388
CC(1/2)	0.993	0.999	0.218

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME