5WHD

Crystal structure of KRas G12V/D38P, bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	37.239, 80.912, 108.041
Unit cell angles	90.00, 90.62, 90.00

Refinement procedure

Resolution	64.762 - 1.641
R-factor	0.194
Rwork	0.192
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gft
RMSD bond length	0.005
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.762	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.067
Number of reflections	74937
<I/σ(I)>	11.5
Completeness [%]	95.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M SODIUM FLUORIDE, 23% PEG 3350,