5WGQ
Estrogen Receptor Alpha Ligand Binding Domain in Complex with Estradiol and SRC2-BCP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.038, 84.044, 58.209 |
| Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
| Resolution | 31.080 - 2.300 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dxe |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.737 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.295 | 2.300 |
| Number of reflections | 21421 | |
| <I/σ(I)> | 0.18 | |
| Completeness [%] | 98.3 | 97.6 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | PEG 3,350, Tris pH 8.5, MgCl2 |
