5WGK

Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 in complex with HPB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 2 21 21
Unit cell lengths	51.670, 83.970, 94.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.883 - 1.822
R-factor	0.1502
Rwork	0.149
R-free	0.17310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eem
RMSD bond length	0.011
RMSD bond angle	0.855
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.790
High resolution limit [Å]	1.820	1.820
Number of reflections	37115
<I/σ(I)>	11.7
Completeness [%]	99.1
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	ammonium iodide, PEG3350