5WG5
Human GRK2 in complex with Gbetagamma subunits and CCG224061
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.1271 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.022, 240.130, 211.611 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.980 - 3.100 |
R-factor | 0.1898 |
Rwork | 0.188 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pnk |
RMSD bond length | 0.002 |
RMSD bond angle | 0.452 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 28709 |
<I/σ(I)> | 20.8 |
Completeness [%] | 98.0 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES pH 6, 8-15% PEG3350, 0.8-1.2 M NaCl |