5WG3
Human GRK2 in complex with Gbetagamma subunits and CCG258748
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.267, 241.569, 214.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.071 - 2.896 |
| R-factor | 0.2009 |
| Rwork | 0.198 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pnk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.556 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.896 | 2.896 |
| Rmerge | 0.132 | 0.855 |
| Number of reflections | 35941 | 3411 |
| <I/σ(I)> | 9.35 | 1.3 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 50 mM MES pH 6, 1.1 M NaCl, 8% PEG3350 |
