5WBU

Crystal structure of mTOR(deltaN)-mLST8-PRAS40(alpha-helix & beta-strand) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 2 21 21
Unit cell lengths	139.400, 163.200, 207.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 3.420
R-factor	0.23531
Rwork	0.235
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jsn
RMSD bond length	0.009
RMSD bond angle	1.302
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	3.520
High resolution limit [Å]	3.400	7.320	3.400
Rmerge	0.136	0.040	0.590
Rmeas	0.158	0.045	0.711
Rpim	0.079	0.021	0.386
Total number of observations	227760
Number of reflections	61593		5497
<I/σ(I)>	4.5
Completeness [%]	95.5	97.9	86.5
Redundancy	3.7	4.2	2.8
CC(1/2)		0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000