5W87
Crystal structure of the C-terminal lobe of the human HERC6 HECT domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298.15 |
Detector technology | CCD |
Collection date | 2017-03-27 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 |
Unit cell lengths | 74.579, 74.579, 114.890 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.879 - 2.199 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.24350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c4z |
RMSD bond length | 0.002 |
RMSD bond angle | 0.528 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.880 | 2.278 |
High resolution limit [Å] | 2.199 | 2.199 |
Rmerge | 0.057 | 0.758 |
Rpim | 0.035 | 0.623 |
Number of reflections | 11479 | 782 |
<I/σ(I)> | 12.12 | |
Completeness [%] | 91.8 | 64.41 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | K2HPO4/NaH2PO4, NaCl, imidazole:HCl |