5W87

Crystal structure of the C-terminal lobe of the human HERC6 HECT domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298.15
Detector technology	CCD
Collection date	2017-03-27
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	74.579, 74.579, 114.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.879 - 2.199
R-factor	0.2129
Rwork	0.211
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c4z
RMSD bond length	0.002
RMSD bond angle	0.528
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.880	2.278
High resolution limit [Å]	2.199	2.199
Rmerge	0.057	0.758
Rpim	0.035	0.623
Number of reflections	11479	782
<I/σ(I)>	12.12
Completeness [%]	91.8	64.41
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	K2HPO4/NaH2PO4, NaCl, imidazole:HCl