5W5Z

Crystal structure of BAXP168G in complex with an activating antibody at high resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 41 21 2
Unit cell lengths	67.863, 67.863, 264.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.986 - 1.997
R-factor	0.2189
Rwork	0.217
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zan
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2152)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.986
High resolution limit [Å]	1.997	1.997
Rmerge	0.112	1.745
Number of reflections	43112
<I/σ(I)>	14.18
Completeness [%]	100.0
Redundancy	9.5
CC(1/2)	0.999	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Peg4000, MES