5W5K
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 in complex with KV70
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.290, 170.940, 151.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.141 - 2.700 |
R-factor | 0.2077 |
Rwork | 0.204 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eem |
RMSD bond length | 0.003 |
RMSD bond angle | 0.783 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2776: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 87.141 |
High resolution limit [Å] | 2.700 |
Number of reflections | 34541 |
<I/σ(I)> | 5.5 |
Completeness [%] | 95.5 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | magnesium acetate, HEPES (pH 7.5), PEG 8,000 |