5W5K

Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 in complex with KV70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	101.290, 170.940, 151.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.141 - 2.700
R-factor	0.2077
Rwork	0.204
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eem
RMSD bond length	0.003
RMSD bond angle	0.783
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2776: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	87.141
High resolution limit [Å]	2.700
Number of reflections	34541
<I/σ(I)>	5.5
Completeness [%]	95.5
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	magnesium acetate, HEPES (pH 7.5), PEG 8,000