5W10
Lcd1 GAF domain in complex with cAMP ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.190, 119.995, 62.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.340 - 2.150 |
| R-factor | 0.19617 |
| Rwork | 0.195 |
| R-free | 0.22519 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.078 | 0.560 |
| Number of reflections | 93522 | |
| <I/σ(I)> | 17.8 | 1.6 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M sodium citrate and 20% PEG 3350 |
