5VZJ

STRUCTURE OF A TWELVE COMPONENT MPP6-NUCLEAR RNA EXOSOME COMPLEX BOUND TO RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	141.090, 213.592, 225.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.247 - 3.300
R-factor	0.2192
Rwork	0.217
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4001 5k36
RMSD bond length	0.001
RMSD bond angle	0.398
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.247	150.000	3.420
High resolution limit [Å]	3.300	7.110	3.300
Rmerge	0.086	0.043	0.617
Rmeas	0.101	0.049	0.754
Rpim	0.051	0.024	0.424
Total number of observations	343880
Number of reflections	101546
<I/σ(I)>	8.6
Completeness [%]	97.3	98.8	94.7
Redundancy	3.4	4.1	2.6
CC(1/2)		0.996	0.142

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	11-13% PEG3350, 100mM NaCitrate pH 5.6, 7 mM MES pH 6.5, 175-200 mM ammonium sulfate.