5VZJ
STRUCTURE OF A TWELVE COMPONENT MPP6-NUCLEAR RNA EXOSOME COMPLEX BOUND TO RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 141.090, 213.592, 225.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.247 - 3.300 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4001 5k36 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.398 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.247 | 150.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.110 | 3.300 |
| Rmerge | 0.086 | 0.043 | 0.617 |
| Rmeas | 0.101 | 0.049 | 0.754 |
| Rpim | 0.051 | 0.024 | 0.424 |
| Total number of observations | 343880 | ||
| Number of reflections | 101546 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 97.3 | 98.8 | 94.7 |
| Redundancy | 3.4 | 4.1 | 2.6 |
| CC(1/2) | 0.996 | 0.142 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 11-13% PEG3350, 100mM NaCitrate pH 5.6, 7 mM MES pH 6.5, 175-200 mM ammonium sulfate. |
