5VZ4

Receptor-growth factor crystal structure at 2.20 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	I 2 2 2
Unit cell lengths	75.352, 88.768, 121.293
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.630 - 2.200
R-factor	0.1946
Rwork	0.191
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5VZ3 & 2GH0
RMSD bond length	0.015
RMSD bond angle	1.682
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.630	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.085	0.045	0.577
Rmeas	0.094	0.051	0.644
Rpim	0.039	0.022	0.280
Total number of observations	118710
Number of reflections	20379
<I/σ(I)>	7.5
Completeness [%]	97.4	97.8	85.4
Redundancy	5.8	5.5	4.9
CC(1/2)		0.997	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	297	0.1 M Bis-Tris, pH 6.0, 1.5 M ammonium sulfate, 10% w/v ethylene glycol, 30-minute soak in 0.5 M sodium bromide