5VSI
CH1/Ckappa Fab mutant 15.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-12-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.279, 60.610, 86.241 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 19.790 - 1.510 |
| R-factor | 0.1953 |
| Rwork | 0.194 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vr9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.424 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.095 | 0.669 |
| Number of reflections | 73293 | 10568 |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 97.7 | 96.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | see publication |
