5VND
Crystal structure of FGFR1-Y563C (FGFR4 surrogate) covalently bound to H3B-6527
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-11-16 |
| Detector | RAYONIX |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.494, 58.925, 65.935 |
| Unit cell angles | 90.00, 107.59, 90.00 |
Refinement procedure
| Resolution | 99.370 - 2.200 |
| R-factor | 0.18059 |
| Rwork | 0.178 |
| R-free | 0.22333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v05 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.370 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 1.000 | |
| Rpim | 0.532 | |
| Total number of observations | 4591 | |
| Number of reflections | 46172 | 4550 |
| <I/σ(I)> | 8.6 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG10000, 0.1M MES pH6.2, 0.3M Ammonium Sulphate, 5% Ethylene glycol |
