5VLA

Short PCSK9 delta-P' complex with Fusion2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	110
Detector technology	CCD
Collection date	2015-08-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97741
Spacegroup name	P 32 2 1
Unit cell lengths	70.267, 70.267, 157.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.310 - 2.400
R-factor	0.211
Rwork	0.209
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nmx
RMSD bond length	0.009
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.310	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	16868
<I/σ(I)>	22.3	5.4
Completeness [%]	92.1	100
Redundancy	7.6	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	calcium acetate, PEG 6000