5VHE
DHX36 in complex with the c-Myc G-quadruplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.526, 79.282, 212.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.641 - 3.793 |
R-factor | 0.2427 |
Rwork | 0.238 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vha |
RMSD bond length | 0.002 |
RMSD bond angle | 0.496 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.641 |
High resolution limit [Å] | 3.793 |
Rmerge | 0.275 |
Rpim | 0.092 |
Number of reflections | 12615 |
<I/σ(I)> | 7.81 |
Completeness [%] | 99.2 |
Redundancy | 9.6 |
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294.15 | 200 mM sodium malonate (pH 7.0) and 25% w/v PEG3350 |