5VG9
Structure of the eukaryotic intramembrane Ras methyltransferase ICMT (isoprenylcysteine carboxyl methyltransferase) without a monobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2015-04-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.640, 123.650, 236.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.198 - 4.000 |
| R-factor | 0.3705 |
| Rwork | 0.370 |
| R-free | 0.38860 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.453 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.198 | 4.070 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.071 | |
| Rpim | 0.012 | 0.341 |
| Number of reflections | 6119 | 309 |
| <I/σ(I)> | 85 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 34.6 | 35.6 |
| CC(1/2) | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 26% PEG400 200 mM CaCl2 50 mM Na acetate, pH 5.5 |
