5VDP
Human cyclic GMP-AMP synthase (cGAS) in complex with 2',3'-cGAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.886, 46.733, 88.165 |
| Unit cell angles | 90.00, 110.45, 90.00 |
Refinement procedure
| Resolution | 32.446 - 2.300 |
| R-factor | 0.1995 |
| Rwork | 0.197 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o67 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | PHENIX (dev_1999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.446 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 37195 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 18-20% PEG 3350, 0.2 M ammonium citrate pH 7 |
