5V85
The crystal structure of the protein of DegV family COG1307 from Ruminococcus gnavus ATCC 29149 (alternative refinement of PDB 3JR7 with Vaccenic acid)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.899, 96.899, 159.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.887 - 2.003 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.19770 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.533 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 83.920 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 58785 |
| <I/σ(I)> | 17.85 |
| Completeness [%] | 99.4 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M SODIUM CHLORIDE, 0.1M PHOSPHATE-CITRATE, 10%PEG3000, PH 4.2 |
