5V6Y

Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer with bound high-affinity and altered selectivity adrenomedullin variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	105
Detector technology	CCD
Collection date	2016-06-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	195.496, 69.436, 200.000
Unit cell angles	90.00, 99.43, 90.00

Refinement procedure

Resolution	45.114 - 2.800
R-factor	0.2131
Rwork	0.212
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rwg
RMSD bond length	0.002
RMSD bond angle	0.441
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.106
Number of reflections	61924	2927
<I/σ(I)>	19.56	1
Completeness [%]	94.3	91.4
Redundancy	6.8	6.8
CC(1/2)		0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	15% PEG3350, 0.1 M Sodium Malonate pH 6.0, 50 mM Potassium/Sodium tartrate, 1% Cadaverine