5V6Y
Crystal structure of the human CLR:RAMP1 extracellular domain heterodimer with bound high-affinity and altered selectivity adrenomedullin variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2016-06-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.496, 69.436, 200.000 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 45.114 - 2.800 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rwg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.106 | |
| Number of reflections | 61924 | 2927 |
| <I/σ(I)> | 19.56 | 1 |
| Completeness [%] | 94.3 | 91.4 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15% PEG3350, 0.1 M Sodium Malonate pH 6.0, 50 mM Potassium/Sodium tartrate, 1% Cadaverine |
