5V4M
Structure of HLA-DR15 with bound alpha3(135-145) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.590, 79.010, 95.821 |
| Unit cell angles | 87.64, 73.29, 89.97 |
Refinement procedure
| Resolution | 48.320 - 2.100 |
| R-factor | 0.2175 |
| Rwork | 0.217 |
| R-free | 0.24400 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.666 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.350 | |
| High resolution limit [Å] | 2.100 | |
| Rmerge | 0.129 | 0.709 |
| Rpim | 0.061 | 0.340 |
| Number of reflections | 106633 | 15528 |
| <I/σ(I)> | 41.65 | 2.3 |
| Completeness [%] | 96.3 | 95.5 |
| Redundancy | 5.4 | 5.3 |
| CC(1/2) | 0.995 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 25% PEG 3350, 0.2M KNO3 and 0.1M Bis-Tris-propane (pH 7.5) |
