5V4B

Crystal structure of the Skp1-FBXW7-DISC1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	I 41 2 2
Unit cell lengths	233.514, 233.514, 108.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.930 - 2.600
R-factor	0.1854
Rwork	0.184
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ovp
RMSD bond length	0.015
RMSD bond angle	1.798
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.930	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.103	0.038	0.605
Total number of observations	279701
Number of reflections	45981
<I/σ(I)>	10.2
Completeness [%]	99.8	99.2	99.4
Redundancy	6.1	6.2	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277.15	100 mM Hepes, 1.2M lithium sulfate