5UY3
Crystal structure of human Fab PGT144, a broadly reactive and potent HIV-1 neutralizing antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.600, 67.550, 69.550 |
| Unit cell angles | 82.04, 74.89, 81.26 |
Refinement procedure
| Resolution | 36.293 - 2.900 |
| R-factor | 0.243 |
| Rwork | 0.241 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u1s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.160 | 0.390 |
| Number of reflections | 21292 | |
| <I/σ(I)> | 5.5 | 1.9 |
| Completeness [%] | 96.6 | 96.9 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | JCSG F8 |
