5UWC

Cytokine-receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 61
Unit cell lengths	106.461, 106.461, 96.116
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	92.200 - 2.400
R-factor	0.21763
Rwork	0.216
R-free	0.24199
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jzj 1jli
RMSD bond length	0.012
RMSD bond angle	1.550
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.200	2.400
High resolution limit [Å]	2.400	2.390
Rmerge	0.080
Number of reflections	23081
<I/σ(I)>	19.7
Completeness [%]	99.0
Redundancy	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	20% PEG 8000, 200 mM NaCl and 100 mM citrate-phosphate buffer pH 4.8