5UT0
JAK2 JH2 in complex with AT9283
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-14 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.135, 57.681, 60.443 |
| Unit cell angles | 90.00, 109.95, 90.00 |
Refinement procedure
| Resolution | 41.486 - 2.102 |
| R-factor | 0.1706 |
| Rwork | 0.168 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.486 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 16717 | |
| <I/σ(I)> | 8.9 | 1.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris pH 8.0 0.2M Sodium acetate 12-20% PEG 4,000 |
